Part:BBa_K115030:Experience
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Applications of BBa_K115030
These constructs were used to test our RNA thermometers, by measuring luciferase activity after growing at certain temperatures. The fold increase of luciferase activity between cultures of cells grown at 20ºC and 37ºC is displayed in figure 1.
In this figure, 12 (the leftmost) is construct K115012, the reference strain, which has no thermosensitive RNA, but just B0032 as ribosome binding site. This is to compare for normal temperature induced behaviour in cells. The other constructs from 29-36 are K115029 to K115036.
For more documentation, visit our wiki [http://2008.igem.org/Team:TUDelft (link)].
User Reviews
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